Pentelute Lab MIT | Nitrogen Arylation for Macrocyclization of Unprotected Peptides
We describe an efficient and mild method for the synthesis of macrocyclic peptides via nitrogen arylation from unprotected precursors. Various electrophiles and lysine-based nucleophiles were investigated and showed high-yielding product formation, even for a macrocyclization scan with 14 variants. We found that nitrogen-linked aryl products were more stable to base and oxidation when compared to thiol arylated species, thereby highlighting the utility of this methodology. Finally, N-aryl macrocyclization was performed on a p53 peptide inhibitor of MDM2 and resulted in identification of a nanomolar binder with improved proteolytic stability and cell permeability.
Pentelute Lab, MIT, Publications,
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Nitrogen Arylation for Macrocyclization of Unprotected Peptides

Nitrogen Arylation for Macrocyclization of Unprotected Peptides

J. Am. Chem. Soc., 2016, 138 (27), pp 8340–8343
DOI: 10.1021/jacs.6b03757
Publication Date (Web): June 22, 2016

Guillaume Lautrette, Fayçal Touti, Hong Geun Lee, Peng Dai, and Bradley L. Pentelute*

Abstract

We describe an efficient and mild method for the synthesis of macrocyclic peptides via nitrogen arylation from unprotected precursors. Various electrophiles and lysine-based nucleophiles were investigated and showed high-yielding product formation, even for a macrocyclization scan with 14 variants. We found that nitrogen-linked aryl products were more stable to base and oxidation when compared to thiol arylated species, thereby highlighting the utility of this methodology. Finally, N-aryl macrocyclization was performed on a p53 peptide inhibitor of MDM2 and resulted in identification of a nanomolar binder with improved proteolytic stability and cell permeability.

Category
2016, Publications