Flow-Based Enzymatic Ligation via Sortase A
Policarpo RL, Kang H, Liao X, Rabideau AE, Simon MD, Pentelute BL., Angew Chem Int Ed Engl. 2014 Aug 25;53(35):9203-8
Online July 2, 2014
Sortase-mediated ligation (sortagging) is a versatile, powerful strategy for protein modification. Because the sortase reaction reaches equilibrium, a large excess of polyglycine nucleophile is often employed to drive the reaction forward and suppress sortase-mediated side reactions. A flow-based sortagging platform employing immobilized sortase A within a microreactor was developed that permits efficient sortagging at low nucleophile concentrations. The platform was tested with several reaction partners and used to generate a protein bioconjugate inaccessible by solution-phase batch sortagging.